VENA8 ENDOTHELIAL+微流體芯片的主要特點(diǎn)：
用于細(xì)胞滾動(dòng)、粘附分子研究或細(xì)胞間相互作用研究
適用于各種細(xì)胞懸浮液(原代和細(xì)胞株)包括T細(xì)胞、單核細(xì)胞、外周血單個(gè)核細(xì)胞、中性粒細(xì)胞、嗜酸性粒細(xì)胞、血小板和全血(肝素化)。
Vena8熒光+ TM和 Vena8TM：可以用標(biāo)準(zhǔn)移液器將***系列的粘附因子包覆毛細(xì)管外壁。不同的黏附分子包括VCAM(血管細(xì)胞粘附因子),ICAM(細(xì)胞間粘附因子),MAdCAM，纖維連接蛋白，vWF(人血管性血友病因子/瑞斯托霉素輔因子)、膠原蛋白、纖維蛋白原等。
Vena8內(nèi)皮細(xì)胞+ TM和VenaECTM：容易種植和培養(yǎng)各種內(nèi)皮細(xì)胞(層)包括HUVEC(人臍靜脈血管內(nèi)皮細(xì)胞)，HMVEC(人微血管內(nèi)皮細(xì)胞)，HCAECs(人冠狀動(dòng)脈內(nèi)皮細(xì)胞)等等。
生物芯片具有良好的光學(xué)特性，可在顯微鏡下進(jìn)行清晰的觀察和進(jìn)***步的研究。
剪切應(yīng)力范圍：0.05-20 dyne/ cm2，可以通過(guò)MirusTM Nanopump精確控制。
剪切應(yīng)力的大小及連續(xù)變化的參數(shù)可以設(shè)置。
可在流動(dòng)狀態(tài)下實(shí)時(shí)成像。
The Vena8 Endothelial+? biochip is ideal for cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow. Ideal for use with PCR. Compatible with brightfield, phase contrast imaging and fluorescence microscopy.
Features:
Cell-cell rolling and adhesion assays on cell monolayers and cell culture under shear flow.
Cells cultured within the biochip channels may be retrieved post-assay for subsequent studies (up to 320,000 cells); e.g. PCR. Substrate is removed from biochip and cells are scraped off.
Suitable for whole blood, primary cells, rare cells e.g. where samples are more difficult to retrieve or culture or only small sample volumes available.
No assembly of the biochip is required unlike many standard perfusion chambers / flow chambers.
No luer lock connections which increase dead volume. Cellix's biochips have a unique plug & play connection with tubing connections which are autoclaveable and reuseable. Dead volume is at input port ~ 0.1μL.
Applications: Flow assays using minimal sample volumes (whole blood, cell suspensions, proteins etc.); brightfield, phase contrast and fluorescence microscopy.
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